You would be counting all day and the CFUs overlap and it s a mess.
Remember, keep going until you re very comfortable with the calculations Oh, and do yourself a favor, use a calculator.
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Another hint : Multiply by the reciprocal of the dilution factor one more hint : Don t forget that we re only reporting 2 significant digits.If you want a printer-friendly version of this module, you can find it here in the tale of two lovers pdf a Microsoft Word document.Multiplying by the reciprocal of your sample size is the heart of counting bacteria by the serial diluation method.So, now we finally have all the pieces of serial dilution assembled: Dilute Calculate dilution factor Plate Repeat To make things easier, the standard operating procedure is to go by factors of 10, and to do about 5 or 6 plates altogether.
Let s say I guesstimate that I have about 42 million CFUs per mL - I might want to start with a first dilution of about 1/1000, and plate that.
In order to get a 0,000 dilution, you need to put 1 mL into _ mLs of water.
Oct 19, 2009 Fifth video in a series of videos discussing concentration calculations commonly used in a laboratory.
Every time we dilute, we ll also make a new plate to incubate.But why does it work.I think I have the answer:.I need a hint : Remember you have basically counted 1/10,000th of the original bacteria.When I add a small amount of the concentrated stuff to an empty container, top it off with saline/water/whatever, then remove photoshop cs5 para mac crackeado some small amount, this constitutes a dilution.After that we could do standard 1/10th dilutions.So we might do 5 dilutions and grow up 5 plates.